Frequently asked questions

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What conditions should I use to HPLC purify my oligos?

IDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for an RP-HPLC protocol is:

  • Hamilton PRP-1 Reverse Phase column 250 x 10 mm
  • Buffer A = 100% ACN (acetonitrile)
  • Buffer B = ACN/0.1 M TEAA pH 7.3
  • Run at 4 mL/min over 30 min from 95–60% A
  • Monitor at 260/297 nm

Categories:
Custom DNA & RNA Optional services

Tags:
oligonucleotide primers purification