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We recommend using a buffer made of 10 mM Tris, 0.1 mM EDTA, and 100 mM NaCl, pH 8.0 (such as the IDT NGS Adapter Buffer, Cat. No. 10006743) to dilute the xGen Stubby Adapter for DNBSEQ. Keeping the adapters duplexed requires a buffer with a high salt concentration.