Do the positive and negative controls in the TriFECTa kit come with primers and RT-PCR materials?

The kit does not include primers or PCR reagents for quantification of gene silencing. Separately, we offer primer sets for targeting human or mouse HPRT for use in SYBR® Green–based qPCR assays to monitor RNAi function following transfection with the HPRT-S1 Positive Control DsiRNA.

For other human, mouse, or rat targets, consider using Predesigned PrimeTime™ qPCR Sequences . For additional species, you can easily design a custom PrimeTime qPCR Assay using our free, online PrimerQuest™ Tool.

The TriFECTa Kit includes:

• 3 target-specific Dicer-Substrate siRNAs (DsiRNAs, 2 nmol each)
• 1 negative control DsiRNA (NC1, which does not target any human, mouse, or rat sequence)
• 1 positive control DsiRNA (HPRT-S1 DS Positive Control)
• 1 fluorescent transfection control DsiRNA (TYE 563-labeled transfection control)
• Nuclease-Free Duplex Buffer

IDT recommends that the qPCR assay be located close to site of the DsiRNA target sites to avoid measuring a cleaved transcript that has a remaining fragment.